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eagle-i ID


Resource Type

  1. Induced pluripotent stem cell line


  1. Source Developmental Stage
    Late adult stage
  2. Source Human Subject
  3. Exchange facilitator
    <html> <a href=http://ccr.coriell.org/Sections/Search/Sample_Detail.aspx?Ref=GM23338&PgId=166><img src="http://ccr.coriell.org/Images/c_logo.jpg"></a> </html>
  4. Induction Reagents
  5. Karyotype Description
  6. Resource Description
    Induced pluripotent stem cell line; Participant #1 (hu43860C) in the Personal Genome Project: http://www.personalgenomes.org Fibroblast culture from this same subject from which this cell line was made is GM23248and a matching lymphoblast line from this same subject is GM20431. -- APPARENTLY HEALTHY NON-FETAL TISSUE. The cell line submitted to the Repository frozen was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and directed differentiation toward cardiac neuronal and pancreatic lineages. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. The line was evaluated for in vivo pluripotency via teratoma formation assay. Characterization data are included in the Certificate of Analysis.
  7. Access Restriction(s)
    MTA required
  8. Related Publication or Documentation
  9. Website(s)
  10. Source Anatomical Structure
    Zone of skin
  11. Source Organism Type
    Homo sapiens
  12. Catalog Number
  13. Subject Age at Sampling
  14. Location
    NIGMS Repository (@Coriell)
Provenance Metadata About This Resource Record

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016