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Mouse Metabolic and Phenotyping Core

Directors: Reynolds, Corey L., Ph.D.; Saha, Pradip, Ph.D.

Summary:

The Mouse Metabolic and Phenotyping Core is a comprehensive fee-for-service rodent phenotyping core that contains a multitude of testing capabilities for the assessment of rodent models from embryo to adult. Access to the MMPC is open to all Baylor and non-affiliated Baylor investigators. We provide investigators with a wide variety of state of the art equipment and techniques with the goal to standardize key methodologies, and to expedite comprehensive research analyses on diseases related to cancer, cardiovascular dysfunction, metabolic disorders, mouse models of human disease and drug studies. The MMPC supports the application of advanced analysis of metabolic pathways and related physiological and biochemical parameters in mice and rats, as well as in isolated / cultured cells in vitro. Assays offered include hyperinsulinemic-euglycemic clamp in conscious mice, oxygen consumption rate and extra cellular acidification rate measurements from cells in culture plates, DEXA and Echo MRI, CLAMS, heat and real time body temperature monitoring, home cage activity monitoring, MRI, CT, ultrasound, Xray, bioluminescence imaging, indirect calorimetry, telemetry, and non-invasive blood pressure measurements.

Affiliations:

People:

Resources:

Instruments

  • 16-metabolic cages ( Animal cage )

  • 7.0T Pharmascan MRI ( MRI Scanner )

  • Blood Pressure and ECG Telemetry ( Telemetry system )

  • Comprehensive Lab Animal Monitoring System (CLAMS) ( Animal physiology monitoring system )

    "Columbus Instruments Comprehensive Lab Animal Monitoring System CLAMS is the premiere system of its kind. Incorporating sub-systems for open circuit calorimetry, activity, body mass, feeding, drinking, food access control, running wheel, urine collection, sleep detection, body core temperature and heart rate in an optional environmental chamber: Oxymax/CLAMS is the one-test solution for simultaneous multi parameter assessment of 1 to 32 test animals.

    Operation of Oxymax/CLAMS and data collection is performed by an integrated program. The resulting secure data sets can be exported to Comma Separated Value (CSV) files and provide the link between Oxymax/CLAMS and your existing data analysis tools. Additional data reduction tools allow for the de-collating of data by parameter to facilitate analysis from a parameter perspective. All data can be reviewed graphically & numerically in real-time during collection. "

  • ECG mouse monitor ( ECG monitor )

  • EchoMRI-100™ ( Body composition analyzer )

  • eXplore CT 120 ( Computed tomography scanner )

  • Faxitron Body Composition ( Body composition analyzer )

  • Grip strength meter ( Animal physiology monitoring system )

  • Ms-FX Pro Optical Imager and X-Ray ( In vivo imaging system )

  • Non-Invasive Blood Pressure ( Blood pressure monitor )

    "The IITC method requires minimal warming of rats and mice. The rodents should be warmed to an ambient temperature of 30 deg. c. for rats and 32 deg. c. for mice, which is well below the thermostress level (see NIBP Intro. page). It is suggested to use one of the IITC warming chambers. The artifact filter eliminates the breathing and movement artifacts, enabling the user to pick out endpoints easily. In addition the amplifier has a built in cuff pump which allows for automatic cuff inflation. The built-in cuff pump has adjustable cuff pressure limits with automatic cut off, remote trigger capability, adjustable deflation rate, a manual air release for an immediate pressure drop and a test termination pressure control allows for quick 10 to 15 second test cycles."

  • Oxymax FAST Indirect Calorimetry System ( Animal activity monitoring system )

  • Running wheel ( Exercise equipment )

  • Six lane rodent treadmill ( Treadmill )

    Features adjustable speed, inclination and shock plates.

  • VersaMax system ( Animal activity monitoring system )

  • VisualSonics Vevo 2100 ultrasound ( Ultrasound machine )

    This is a high-resolution instrument (30 microns) that can also image at high speed (up to 100 frames per second). It is capable of imaging in 3D mode as well as M mode, and can also provide pulsed wave Doppler imaging. The device is equipped with two transducers (25 and 40 MHz) and the Vevo Integrated Rail System, which facilitates alignment of the mouse and the probe as well as image-guided injection, when necessary. The table is heated and includes sensor pads for monitoring the animal during examination. The system is routinely used for cardiac imaging in small animals.

  • XF 96 Seahorse Analyzer ( Cell assay platform )

    "The Seahorse XFe96 Analyzer measures OCR and ECAR of live cells in a 96-well plate format. These rates are key indicators of mitochondrial respiration and glycolysis and provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples."

Services


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Last updated: 2018-08-22T14:32:04.002-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016