eagle-i eagle-i Network Shared Resource Repositoryeagle-i Network Shared Resource Repository
See it in Search

Topol Lab

Summary:

My research is on indvidualized medicine, using the genome and digital technologies to understand each person at the biologic, physiologic granular level to determine appropriate therapies and prevention. An example is the use of pharmacogenomics and our research on clopidogrel (Plavix). By determining the reasons for why such a large proportion of people do not respond to this medication, we can use alternative treatment strategies to prevent blood clots.

Affiliations:

People:

Resources:

Human Studies

  • ELUCIDATING CARDIOVASCULAR PHENOTAYPES EMPLOYING GENOME EDITING OF IPS CELLS ( Human Study )

    We aim to develop an innovative approach to generate, at high-throughput, isogenic induced pluripotent stem cells (iPSCs), and use their differentiated progeny to understand the impact of human genetic variation on the risk of developing coronary artery disease (CAD). A genomic variant associated with CAD, myocardial infarction (Ml), abdominal aortic aneurysm, and intracranial aneurysm is found in a stretch of chr9p21 devoid of known genes. We will use this locus as a model for our study; while focusing on 9p21, our overall approach will be broadly applicable to the study of HLBS diseases of complex genetic architecture.

Reagents

  • SCRP0104i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP0202i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP0203i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP0302i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP0307i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP0402i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP0404i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP0517i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP0601i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP0608i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP0709i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP0802i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP1002i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP1005i ( Induced pluripotent stem cell line )

  • SCRP1021i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP1031i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP1041i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP1103i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP1203i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP1307i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP1602i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP1702i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2001i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP2101i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2102i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP2106i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2115i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2201i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2208i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2210i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2211i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2305i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP2306i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP2307i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP2310i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP2407i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2409i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2411i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2501i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2503i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP2506i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP2508i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP2706i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP2801i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP3202i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP3304i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP3401i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP3510i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP3601i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP3702i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP3803i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP3901i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP4102i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP4201i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP4203i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor had a mix of genotypes at the 3 sites.

  • SCRP4301i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP4305i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP4403i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP4406i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP4502i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP4505i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP4602i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP4603i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP4703i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP4803i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP4903i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP5003i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP5402i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP5508i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP5603i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP5707i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP5803i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP6007i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP6101i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP6301i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP6401i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP6504i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP6703i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP6904i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP7301i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP7602i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP7803i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP7903i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP8105i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP8305i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP8401i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP8503i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP8601i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP8717i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the risk alleles at all 3 sites (R/R).

  • SCRP8901i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP9001i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP9301i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP9403i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was homozygous for the non-risk alleles at all 3 sites (NR/NR).

  • SCRP9501i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP9602i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP9805i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

  • SCRP9904i ( Induced pluripotent stem cell line )

    Three sites within the 9p21 region were genotyped for each donor. This donor was heterozygous for the risk and non-risk alleles at all 3 sites (R/NR).

Resource Collection


Web Links:

Last updated: 2016-09-19T12:37:53.265-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016