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KeraFAST

Summary:

Reagents for the Greater Good
Our mission is to grow and foster a global community of scientists advancing life science research by facilitating access to bioresearch materials directly from the scientific community.

We are committed to reinvesting back into research by returning a portion of the sale to the scientists and their institutions that contribute Reagents for the Greater Good. We encourage the community to further scientific progress by providing or procuring novel bioresearch materials, which allows scientists to accelerate their own research and enable other researchers to access materials they need. Since any Procurer can also be a Provider, the entire interconnected community benefits the Greater Good of Science.

How it Works
These rare and often one-of-a-kind research materials are not developed or manufactured by Kerafast, but produced by principal investigators in the course of their work. These materials may be in limited supply, but may be just what your experiments require, and we expedite their delivery through a rapid online material transfer ‘click license’ agreement for research use.

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    Reagents

    • Angelman Syndrome [AG1-0] Human Induced Pluripotent Stem Cells ( Induced pluripotent stem cell line )

      Source Tissue: Fibroblasts
      Cell Line Name: AG1-0
      Biosafety Level: 2
      Growth Medium Conditions: hESC medium (DMEM/F12 with 20% Knockout serum replacer, 1X Non-essential amino acids, 1mM L-glutamine, 4ug/mL basic Fibroblast growth factor)
      Growth Properties: Prefers to grow in colonies, feed daily, split once per week
      Sub-culturing: Manually split no more than 1:5, depending on density of dish
      Cryo-preservation Medium: 60% DMEM/F12, 30% FBS, 10% DMSO
      Storage Temperature: Cells should be cultured at 37C upon arrival. Cryopreserved cells should be stored in Liquid Nitrogen
      Shipped: Shipped at room temperature as live cells in T25 flask

      These cells have been propagated using irradiated mouse embryonic fibroblasts as a feeder layer. It is recommended to keep the cells on a feeder layer until established.

    • Prader-Willi Syndrome [PWS1-7] Human Induced Pluripotent Stem Cells ( Induced pluripotent stem cell line )

      Source Tissue: Fibroblasts
      Cell Line Name: PWS1-7
      Biosafety Level: 2
      Growth Medium Conditions: hESC medium (DMEM/F12 with 20% Knockout serum replacer, 1X Non-essential amino acids, 1mM L-glutamine, 4ug/mL basic Fibroblast growth factor)
      Growth Properties: Prefers to grow in colonies, feed daily, split once per week
      Sub-culturing: Manually split no more than 1:5, depending on density of dish
      Cryo-preservation Medium: 60% DMEM/F12, 30% FBS, 10% DMSO
      Storage Temperature: Cells should be cultured at 37C upon arrival. Cryopreserved cells should be stored in Liquid Nitrogen
      Shipped: Shipped at room temperature as live cells in T25 flask

      These cells have been propagated using irradiated mouse embryonic fibroblasts as a feeder layer. It is recommended to keep the cells on a feeder layer until established.


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    Last updated: 2014-12-15T09:15:55.632-06:00

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    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016