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NINDS biorepository (NHCDR @Rutgers)

Summary:

NINDS fibroblast and iPSC cell lines previously available from the NINDS Repository at Coriell can now be ordered through the NINDS Human Cell and Data Repository (NHCDR) at Rutgers.

Cell line requests that were received prior to the transition will be fulfilled by the NHCDR beginning the week of April 25th, 2016.

If cell lines were previously received from Coriell and the recipient is now requesting permission to distribute these lines to a third party, please forward these requests to NINDS@dls.rutgers.edu.

For specific details about existing requests please contact Dr. Michael Sheldon sheldon@dls.rutgers.edu.

"The NINDS Repository offers human induced pluripotent stem cell (iPSC) lines to further research on neurological disorders. The iPSC lines were derived from fibroblasts using a variety of reprogramming methods and submitted to the NINDS Repository. Each iPSC line is accompanied by a Certificate of Analysis prepared by the Coriell SCB."

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  • ND29149-Fibroblast ( Cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND29422-Fibroblast ( Cell line )

  • ND29509-Fibroblast ( Cell line )

  • ND29774-Fibroblast ( Cell line )

  • ND34391-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND34393-iPSC ( Induced pluripotent stem cell line )

    same subject as ND34394 (iPSC sub-clone) and ND32320 (whole-blood derived DNA; Cells were cultured in DMEM/F12 supplemented with 20% KOSR, 2 mM L-glutamine, 0.1 mM Non-essential amino acids, 0.1 mM 2-mercaptoethanol and bFGF (concentration stated on Certificate of Analysis) and plated on gelatin coated plates using irradiated CF-1 mouse embryonic fibroblasts as feeder layer.

  • ND34394-iPSC ( Induced pluripotent stem cell line )

    same subject as ND34394 (iPSC sub-clone) and ND32320 (whole-blood derived DNA; Cells were cultured in DMEM/F12 supplemented with 20% KOSR, 2 mM L-glutamine, 0.1 mM Non-essential amino acids, 0.1 mM 2-mercaptoethanol and bFGF (concentration stated on Certificate of Analysis) and plated on gelatin coated plates using irradiated CF-1 mouse embryonic fibroblasts as feeder layer.

  • ND35367-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35371-iPSC ( Induced pluripotent stem cell line )

    ASYMPTOMATIC OR UNDIAGNOSED AND GENETICALLY RELATED TO AN AFFECTED INDIVIDUAL. same subject as ND32943 and ND32386

  • ND35658-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35659-iPSC ( Induced pluripotent stem cell line )

    ASYMPTOMATIC OR UNDIAGNOSED AND GENETICALLY RELATED TO AN AFFECTED INDIVIDUAL

  • ND35660-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35661-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35662-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35663-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35664-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35666-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35668-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35669-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35670-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35671-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND35673-iPSC ( Induced pluripotent stem cell line )

    ASYMPTOMATIC OR UNDIAGNOSED AND GENETICALLY RELATED TO AN AFFECTED INDIVIDUAL

  • ND36997-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND36998-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND36999-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND38477-iPSC ( Induced pluripotent stem cell line )

    PARKINSON DISEASE. The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. 1-BP DEL, 255A; In 2 Spanish families, Hoenicka et al. (2002) found deletion of 1 adenine at nucleotide 202 of the PARK2 gene in members with juvenile Parkinson disease (600116). The mutation was homozygous in 1 family and compound heterozygous with a deletion of exons 8 and 9 (602544.0006) in the other. In the first family, there was 1 individual who was a heterozygous carrier of only the 255delA mutation who developed transient drug-induced parkinsonism at 45 years of age while being treated with haloperidol. The 255delA mutation in PARK2 was originally described by Abbas et al. (1999).

  • ND38545-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND38546-iPS ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND38547-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND38548-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND38549-iPSC ( Induced pluripotent stem cell line )

    Clinically unaffected female

  • ND38551-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND38552-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND38554-iPSC ( Induced pluripotent stem cell line )

    unaffected; last examined on 3/27/81; affected sibs are GM04287 & GM04285; father is GM04815; same subject as GM04729 and GM04730A

  • ND38555-iPSC ( Induced pluripotent stem cell line )

    Code 425; unaffected; last examined on 3/27/81; affected sibs are GM04799, 05621Z, & 05613Z; same subject as GM04797 and GM04796

  • ND39027-Fibroblast ( Cell line )

  • ND39032-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND39034-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND39036-iPSC ( Induced pluripotent stem cell line )

    ASYMPTOMATIC OR UNDIAGNOSED AND GENETICALLY RELATED TO AN AFFECTED INDIVIDUAL

  • ND39037-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND39896-iPSC ( Induced pluripotent stem cell line )

    PARKINSON DISEASE. No known PD mutation; sporadic PD. Subject with skin cancer, diabetes, ulcerative colitis, hypertension and enlarged prostate.

  • ND40018-iPSC ( Induced pluripotent stem cell line )

    The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND40019-iPSC ( Induced pluripotent stem cell line )

    ASYMPTOMATIC OR UNDIAGNOSED AND GENETICALLY RELATED TO AN AFFECTED INDIVIDUAL. The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND40020-iPSC ( Induced pluripotent stem cell line )

    ASYMPTOMATIC OR UNDIAGNOSED AND GENETICALLY RELATED TO AN AFFECTED INDIVIDUAL. The cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND41113-iPSC ( Induced pluripotent stem cell line )

    ASYMPTOMATIC; HD GENE-NEGATIVE, At Risk, same subject as ND36997(iPSC), GM02182 (lymphoblastoid cell line) and GM02183 (fibroblast); see GM family 110

  • ND41114-iPSC ( Induced pluripotent stem cell line )

    ASYMPTOMATIC; HD GENE-NEGATIVE, Same subject as GM23241

  • ND41656-iPS ( Induced pluripotent stem cell line )

    The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND41657-iPS ( Induced pluripotent stem cell line )

    The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND41865-iPSC ( Induced pluripotent stem cell line )

    POPULATION/CONVENIENCE CONTROL

  • ND41866-iPSC ( Induced pluripotent stem cell line )

    POPULATION/CONVENIENCE CONTROL

  • ND41869-iPSC ( Induced pluripotent stem cell line )

    The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND41870-iPSC ( Induced pluripotent stem cell line )

    The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

  • ND41872-iPSC ( Induced pluripotent stem cell line )

    The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.


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Last updated: 2016-06-22T14:28:41.223-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016