eagle-i eagle-i Network Shared Resource Repositoryeagle-i Network Shared Resource Repository
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.

IPS-DERIVED HEPATOCYTES FOR INTERROGATION OF LIPID PHENOTYPES

eagle-i ID

http://shared.eagle-i.net/i/00000155-9341-0329-dc2e-0c4980000000

Resource Type

  1. Human Study

Properties

  1. Related grant number
    5U01HG006398
  2. Resource Description
    Plasma concentrations of lipids (LDL-C, HDL-C and triglycerides) are important risk factors for atherosclerotic cardiovascular disease and have proven fertile territory for genome-wide association studies (GWAS). The Global Lipids Genetics Consortium, a consortium of lipid GWAS totaling approximately 100,000 subjects, has identified a total of 95 loci that are genome-wide significantly associated with at least one plasma lipid trait. While this list includes most of the genes that are known causes of Mendelian lipid disorders, the majority are not previously associated with lipoprotein metabolism. It is key to determine how these genetic loci affect phenotypes in human tissue types relevant to lipid metabolism, especially the liver. This will lead to a greater understanding of the physiological regulation of lipoprotein metabolism and identify novel therapeutic targets for reducing LDL-C and TG and raising HDL-C. There is a need to establish infinitely renewable sources of functional hepatocytes from patients with defined genotype in order to study the cellular mechanisms by which genotype influences hepatocyte lipid biology and plasma lipid traits. We have developed the ability to use adipose stem cells (ASCs) to serve as the basis for obtaining reprogrammed induced pluripotent stem (iPS) cells and have a core dedicated to both ASC isolation and to scaling up iPS generation. Through collaboration between Penn and Medical College of Wisconsin we have established protocols for the differentiation of human iPS cells into hepatocytes. Our consortium comprises investigators expert in iPS generation, hepatocyte generation, and hepatocyte lipid metabolism. We will develop a library of iPS cell lines and iPS-derived hepatocytes from ~300 hundred subjects with defined genotypes and will use them to (1) develop highly efficient and reliable protocols to obtain iPS cell lines from ASCs, followed by differentiation into functional hepatocytes; (2) scale up these protocols to enable high- throughput generation of iPS cell lines and hepatocytes; and (3) perform metabolic profiling from these hepatocytes. These studies should provide novel insights into the molecular mechanisms by which some of the most compelling GWAS loci for plasma lipid traits influence lipoprotein metabolism.
  3. Contact
    Rader, Daniel, Ph.D.
  4. PI
    Rader, Daniel, Ph.D.
  5. Topic
    atherosclerosis
  6. Website(s)
    https://projectreporter.nih.gov/project_info_description.cfm?aid=8889285&icde=30085197
  7. Funded by
    National Human Genome Research Institute
  8. Related Cell Line
    Penn056i-1-1
  9. Related Cell Line
    Penn144i-M7-16
  10. Related Cell Line
    Penn150i-M7-9
  11. Related Cell Line
    Penn073i-133-8
  12. Related Cell Line
    Penn033i-182-2
  13. Related Cell Line
    Penn111i-222-5
  14. Related Cell Line
    Penn101i-263-39
  15. Related Cell Line
    Penn004i-277-1
  16. Related Cell Line
    Penn112i-304-6
  17. Related Cell Line
    Penn103i-330-4
  18. Related Cell Line
    Penn105i-342-5
  19. Related Cell Line
    Penn076i-361-2
  20. Related Cell Line
    Penn110i-389-6
  21. Related Cell Line
    Penn082i-562-6
  22. Related Cell Line
    Penn122i-627-5
  23. Related Cell Line
    Penn026i-124-1
  24. Related Cell Line
    Penn097i-17-1
  25. Related Cell Line
    Penn137i-18-11
  26. Related Cell Line
    Penn072i-187-3
  27. Related Cell Line
    Penn027i-40-2
  28. Related Cell Line
    Penn008i-77-5
  29. Related Cell Line
    Penn128i-8-4
  30. Related Cell Line
    Penn006i-149-1
  31. Related Cell Line
    Penn119i-197-25
  32. Related Cell Line
    Penn099i-218-1
  33. Related Cell Line
    Penn053i-292-3
  34. Related Cell Line
    Penn028i-637-2
  35. Related Cell Line
    Penn017i-638-2
  36. Related Cell Line
    Penn021i-749-4
  37. Related Cell Line
    Penn095i-123-7
  38. Related Cell Line
    Penn116i-125-16
  39. Related Cell Line
    Penn045i-25-12
  40. Related Cell Line
    Penn001i-87-2
  41. Related Cell Line
    Penn151i-M1-5
  42. Related Cell Line
    Penn149i-M1-6
  43. Related Cell Line
    Penn133i-252-23
  44. Related Cell Line
    Penn075i-309-1
  45. Related Cell Line
    Penn140i-M12-7
  46. Related Cell Line
    Penn041i-177-46
  47. Related Cell Line
    Penn015i-668-5
  48. Related Cell Line
    Penn029i-752-3
  49. Related Cell Line
    Penn152i-M18-1
  50. Related Cell Line
    Penn154i-M18-2
  51. Related Cell Line
    Penn100i-623-3
  52. Related Cell Line
    Penn071i-216-13
  53. Related Cell Line
    Penn081i-352-4
  54. Related Cell Line
    Penn035i-746-3
  55. Related Cell Line
    Penn121i-69-1
  56. Related Cell Line
    Penn079i-33-1
  57. Related Cell Line
    Penn172i-M15-10
  58. Related Cell Line
    Penn166i-M15-4
  59. Related Cell Line
    Penn163i-M4-4
  60. Related Cell Line
    Penn141i-M6-2
  61. Related Cell Line
    Penn160i-M6-6
  62. Related Cell Line
    Penn148i-M8-3
  63. Related Cell Line
    Penn156i-M8-2
  64. Related Cell Line
    Penn074i-415-3
  65. Related Cell Line
    Penn127i-415-1
  66. Related Cell Line
    Penn057i-427-3
  67. Related Cell Line
    Penn052i-444-2
  68. Related Cell Line
    Penn120i-444-1
  69. Related Cell Line
    Penn084i-555-2
  70. Related Cell Line
    Penn059i-555-1
  71. Related Cell Line
    Penn091i-588-2
  72. Related Cell Line
    Penn020i-588-6
  73. Related Cell Line
    Penn016i-821-1
  74. Related Cell Line
    Penn061i-821-2
  75. Related Cell Line
    Penn164i-M11-1
  76. Related Cell Line
    Penn146i-M11-5
  77. Related Cell Line
    Penn062i-278-2
  78. Related Cell Line
    Penn086i-278-1
  79. Related Cell Line
    Penn078i-SV10
  80. Related Cell Line
    Penn123i-SV20
  81. Related Cell Line
    Penn019i-136-2
  82. Related Cell Line
    Penn125i-233-4
  83. Related Cell Line
    Penn042i-258-12
  84. Related Cell Line
    Penn136i-262-1
  85. Related Cell Line
    Penn046i-271-1
  86. Related Cell Line
    Penn058i-285-3
  87. Related Cell Line
    Penn038i-366-6
  88. Related Cell Line
    Penn070i-408-1
  89. Related Cell Line
    Penn077i-521-1
  90. Related Cell Line
    Penn083i-679-4
  91. Related Cell Line
    Penn068i-697-3
  92. Related Cell Line
    Penn032i-15-5
  93. Related Cell Line
    Penn093i-16-7
  94. Related Cell Line
    Penn034i-322-1
  95. Related Cell Line
    Penn005i-35-3
  96. Related Cell Line
    Penn087i-38-1
  97. Related Cell Line
    Penn098i-50-18
  98. Related Cell Line
    Penn043i-633-3
  99. Related Cell Line
    Penn130i-78-3
  100. Related Cell Line
    Penn132i-131-5
  101. Related Cell Line
    Penn040i-134-2
  102. Related Cell Line
    Penn107i-145-1
  103. Related Cell Line
    Penn094i-161-1
  104. Related Cell Line
    Penn050i-245-10
  105. Related Cell Line
    Penn047i-254-60
  106. Related Cell Line
    Penn049i-334-8
  107. Related Cell Line
    Penn002i-442-1
  108. Related Cell Line
    Penn118i-113-2
  109. Related Cell Line
    Penn126i-120-1
  110. Related Cell Line
    Penn124i-28-3
  111. Related Cell Line
    Penn014i-37-3
  112. Related Cell Line
    Penn010i-486-2
  113. Related Cell Line
    Penn018i-487-4
  114. Related Cell Line
    Penn044i-51-1
  115. Related Cell Line
    Penn065i-553-2
  116. Related Cell Line
    Penn031i-56-1
  117. Related Cell Line
    Penn009i-57-52
  118. Related Cell Line
    Penn069i-60-1
  119. Related Cell Line
    Penn013i-72-1
  120. Related Cell Line
    Penn106i-75-39
  121. Related Cell Line
    Penn085i-76-5
  122. Related Cell Line
    Penn023i-82-1
  123. Related Cell Line
    Penn131i-86-3
  124. Related Cell Line
    Penn037i-90-3
  125. Related Cell Line
    Penn102i-96-1
  126. Related Cell Line
    Penn117i-88-32
  127. Related Cell Line
    Penn039i-63-1
  128. Related Cell Line
    Penn096i-44-4
  129. Related Cell Line
    Penn060i-23-1
  130. Related Cell Line
    Penn064i-22-2
  131. Related Cell Line
    Penn114i-127-2
  132. Related Cell Line
    Penn113i-126-66
  133. Related Cell Line
    Penn090i-111-4
  134. Related Cell Line
    Penn051i-108-1
  135. Related Cell Line
    Penn055i-106-2
  136. Related Cell Line
    Penn109i-802-2
  137. Related Cell Line
    Penn108i-781-3
  138. Related Cell Line
    Penn007i-765-3
  139. Related Cell Line
    Penn089i-758-4
  140. Related Cell Line
    Penn011i-719-2
  141. Related Cell Line
    Penn092i-560-3
  142. Related Cell Line
    Penn030i-384-4
  143. Related Cell Line
    Penn024i-370-3
  144. Related Cell Line
    Penn115i-316-1
  145. Related Cell Line
    Penn036i-286-3
  146. Related Cell Line
    Penn054i-225-33
  147. Related Cell Line
    Penn104i-321-6
  148. Related Cell Line
    Penn003i-661-4
  149. Related Cell Line
    Penn088i-854-7
  150. Related Cell Line
    Penn063i-114-1
  151. Related Cell Line
    Penn138i-24-4
  152. Related Cell Line
    Penn135i-30-8
  153. Related Cell Line
    Penn048i-41-6
  154. Related Cell Line
    Penn134i-61-26
  155. Related Cell Line
    Penn022i-89-1
  156. Related Cell Line
    Penn012i-93-2
  157. Related Cell Line
    Penn025i-71-58
  158. Related Cell Line
    Penn129i-20-14
  159. Related Cell Line
    Penn145i-M16-2
  160. Related Cell Line
    Penn169i-M17-3
  161. Related Cell Line
    Penn170i-M17-4
  162. Related Cell Line
    Penn157i-M2-6
  163. Related Cell Line
    Penn142i-M3-19
  164. Related Cell Line
    Penn155i-M3-21
  165. Related Cell Line
    Penn167i-M5-3
  166. Related Cell Line
    Penn139i-M5-4
  167. Related Cell Line
    Penn147i-M9-7
  168. Related Cell Line
    Penn171i-M9-9
  169. Related Cell Line
    Penn162i-M14-11
  170. Related Cell Line
    Penn159i-M14-4
  171. Related Cell Line
    Penn161i-M10-5
  172. Related Cell Line
    Penn143i-M10-11
  173. Performed by
    Rader Lab
  174. Part of Collection
    NHLBI - Next Generation Genetic Association Studies (U01)-RFA-HL-11-006
 
RDFRDF
 
Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016