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Prader-Willi Syndrome [PWS1-7] Human Induced Pluripotent Stem Cells

eagle-i ID


Resource Type

  1. Induced pluripotent stem cell line


  1. Source Human Subject
    PWSdel1-Human Subject
  2. Exchange facilitator
    <a href="http://www.kerafast.com/p-1546-prader-willi-syndrome-pws1-7-human-induced-pluripotent-stem-cells.aspx"><img src="http://www.kerafast.com/App_Themes/Skin_2/images/new-logo-tagline.png"><br>Click here to order</a>
  3. Induction Reagents
    OCT4, SOX2, KLF4, MYC, and LIN28
  4. Karyotype Description
    46 XX
  5. Resource Description
    Source Tissue: Fibroblasts Cell Line Name: PWS1-7 Biosafety Level: 2 Growth Medium Conditions: hESC medium (DMEM/F12 with 20% Knockout serum replacer, 1X Non-essential amino acids, 1mM L-glutamine, 4ug/mL basic Fibroblast growth factor) Growth Properties: Prefers to grow in colonies, feed daily, split once per week Sub-culturing: Manually split no more than 1:5, depending on density of dish Cryo-preservation Medium: 60% DMEM/F12, 30% FBS, 10% DMSO Storage Temperature: Cells should be cultured at 37C upon arrival. Cryopreserved cells should be stored in Liquid Nitrogen Shipped: Shipped at room temperature as live cells in T25 flask These cells have been propagated using irradiated mouse embryonic fibroblasts as a feeder layer. It is recommended to keep the cells on a feeder layer until established.
  6. Additional Name
  7. Access Restriction(s)
  8. Related Disease
    Prader-Willi syndrome
  9. Related Publication or Documentation
    Induced pluripotent stem cell models of the genomic imprinting disorders Angelman and Prader-Willi syndromes
  10. Website(s)
  11. Genetic Alteration(s)
  12. Source Organism Type
    Homo sapiens
  13. Catalog Number
  14. Developed by
  15. Potency
    Germ layer
  16. Potency
  17. Potency
  18. Potency
  19. Parental Line
  20. QC Report
  21. QC Report
    PWS1-7-QC-Embryoid Body analysis
  22. Induction Method
    Genomic integration/retroviral induction
  23. Location
Provenance Metadata About This Resource Record

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