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Prader-Willi Syndrome [PWS1-7] Human Induced Pluripotent Stem Cells

eagle-i ID

http://shared.eagle-i.net/i/00000149-aa96-e0f6-5520-30d080000000

Resource Type

  1. Induced pluripotent stem cell line

Properties

  1. Source Human Subject
    PWSdel1-Human Subject
  2. Exchange facilitator
    <a href="http://www.kerafast.com/p-1546-prader-willi-syndrome-pws1-7-human-induced-pluripotent-stem-cells.aspx"><img src="http://www.kerafast.com/App_Themes/Skin_2/images/new-logo-tagline.png"><br>Click here to order</a>
  3. Induction Reagents
    OCT4, SOX2, KLF4, MYC, and LIN28
  4. Karyotype Description
    46 XX
  5. Resource Description
    Source Tissue: Fibroblasts Cell Line Name: PWS1-7 Biosafety Level: 2 Growth Medium Conditions: hESC medium (DMEM/F12 with 20% Knockout serum replacer, 1X Non-essential amino acids, 1mM L-glutamine, 4ug/mL basic Fibroblast growth factor) Growth Properties: Prefers to grow in colonies, feed daily, split once per week Sub-culturing: Manually split no more than 1:5, depending on density of dish Cryo-preservation Medium: 60% DMEM/F12, 30% FBS, 10% DMSO Storage Temperature: Cells should be cultured at 37C upon arrival. Cryopreserved cells should be stored in Liquid Nitrogen Shipped: Shipped at room temperature as live cells in T25 flask These cells have been propagated using irradiated mouse embryonic fibroblasts as a feeder layer. It is recommended to keep the cells on a feeder layer until established.
  6. Additional Name
    PWS1-7
  7. Access Restriction(s)
    NONPROFIT ONLY
  8. Related Disease
    Prader-Willi syndrome
  9. Related Publication or Documentation
    Induced pluripotent stem cell models of the genomic imprinting disorders Angelman and Prader-Willi syndromes
  10. Website(s)
    http://www.kerafast.com/p-1546-prader-willi-syndrome-pws1-7-human-induced-pluripotent-stem-cells.aspx
  11. Genetic Alteration(s)
    del-15q11.2-q13.1
  12. Source Organism Type
    Homo sapiens
  13. Catalog Number
    ECN002
  14. Developed by
    00000149-aa56-6109-5520-30d080000000
  15. Potency
    Germ layer
  16. Potency
    Ectoderm
  17. Potency
    Endoderm
  18. Potency
    Mesoderm
  19. Parental Line
    00000149-aa99-3277-5520-30d080000000
  20. QC Report
    PWS1-7-QC-Immunostain
  21. QC Report
    PWS1-7-QC-Embryoid Body analysis
  22. Induction Method
    Genomic integration/retroviral induction
  23. Location
    KeraFAST
 
RDFRDF
 
Provenance Metadata About This Resource Record

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016